To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Movie 4 IFT25RNAi cell expressing TdT::IFT140 grown in the presence of tetracycline and hence in RNAi conditions. No trafficking is observed and TdT::IFT140 appears to remain at the base of the flagella. IFT25 is required for the construction of the trypanosome flagellum Diego Huet, Thierry Blisnick, Sylvie Perrot, and Philippe Bastin J Cell Sci 2019. 132:None-None; doi: 10.1242/jcs.228296 Download Original Video