Movie 1

Tpm3.1-APEX2 decorated actin filaments show a striated organization of Tpm3.1/3.2 in stress fibres. Tomogram reconstruction showing the 3D organization of Tpm3.1/3.2 coated actin filaments within a cultured homozygous Tpm3.1-APEX2 +/+ primary MEF. Dark enrichments correspond with Tpm3.1-APEX2 enriched regions (tomogram depth = 200 nm). Scale bar = 200 nm.