Movie 2
Tpm3.1/3.2 coated actin filaments are intact in sections processed for electron microscopy. A movie of showing a tomogram of Tpm3.1/3.2 coated actin filaments within a cultured homozygous Tpm3.1-APEX2 +/+ primary MEF. Dark enrichments correspond with APEX2-Tpm3.1 enriched regions (tomogram depth = 200 nm). Zoom-in isosurface rendering of the selected volume (magenta) highlights the intactness of the actin filaments. Scale bar = 200 nm.
Colocation of Tpm3.1 and myosin IIa heads defines a discrete subdomain in stress fibres
J Cell Sci 2019. 132:None-None; doi: 10.1242/jcs.228916