Tpm3.1/3.2 coated actin filaments are intact in sections processed for electron microscopy. A movie of showing a tomogram of Tpm3.1/3.2 coated actin filaments within a cultured homozygous Tpm3.1-APEX2 +/+ primary MEF. Dark enrichments correspond with APEX2-Tpm3.1 enriched regions (tomogram depth = 200 nm). Zoom-in isosurface rendering of the selected volume (magenta) highlights the intactness of the actin filaments. Scale bar = 200 nm.
Joyce C. M. Meiring, Nicole S. Bryce, Maria Lastra Cagigas, Aleš Benda, Renee M. Whan, Nicholas Ariotti, Robert G. Parton, Jeffrey H. Stear, Edna C. Hardeman, and Peter W. Gunning
J Cell Sci
2019. 132:None-None; doi: 10.1242/jcs.228916