Movie 1

Time-lapse observation of nuclear protein leakage in living cells of the bqt4Δ lem2 shut-off strain.
Movie associated with Fig. 7. Cells of the bqt4Δ lem2 shut-off strain expressing GFP-GST-NLS were cultured in EMMG liquid medium and observed under a fluorescent microscope immediately after addition of thiamine. Images were collected every 5 minutes at 3 focal sections up to 24 hours. Unprocessed single-section images in every time points are shown in the movie. The number at upper-left indicates the elapsed time in hours:minutes.

J Cell Sci ; doi: 10.1242/jcs.229021