Movie 2

ATM inhibitor Ku55933 partially rescues mitotic slippage caused by Nu7026 after 6 Gy, related to Figure 7 and Figure S6.
Time-lapse imaging of MCF7-FUCCI cells treated with Ku55933 (0.3 μM) or the combination of Ku55933 and Nu7026 (3 μM) following 6 Gy. Cells were treated with inhibitors for 1 h, irradiated with 6 Gy and then images were taken at 2 h intervals. In both cases, most surviving cells entered the cell cycle and completed cytokinesis, but many did not progress further. A high proportion of these daughters contained micronuclei and died instead of continuing on to senescence. Scale bar = 100 μm.

Repair-independent functions of DNA-PKcs protect irradiated cells from mitotic slippage and accelerated senescence

Yue Liu, Elena V. Efimova, Aishwarya Ramamurthy, and Stephen J. Kron

J Cell Sci 2019. 132:None-None; doi: 10.1242/jcs.229385