Movie 3

Mitosis and/or cytokinesis defects in unirradiated cells is sufficient to promote accelerated senescence, related to Figure 7 and Figure S7.
Time-lapse imaging of MCF7-FUCCI cells treated with DMSO, Aurora B kinase inhibitor, or PLK-1 inhibitor. Images were taken at 2 h intervals beginning 1 h after inhibitors treatment. With DMSO treatment, most of the cells underwent normal mitosis in which cell divided approximately every 24 h. With Aurora B kinase or PLK-1 inhibitor treatment, cells underwent mitotic slippage and/or catastrophe. Though many cells died, surviving cells eventually displayed senescent phenotype with mCherry-hCdt1 (red, G1) expression. Scale bar = 100 μm.

Repair-independent functions of DNA-PKcs protect irradiated cells from mitotic slippage and accelerated senescence

Yue Liu, Elena V. Efimova, Aishwarya Ramamurthy, and Stephen J. Kron

J Cell Sci 2019. 132:None-None; doi: 10.1242/jcs.229385