Movie 2

CASK shKD#1 cyst, grown on Matrigel, showing a number of abnormally oriented cell divisions; cell division starting at 0 minutes, corresponds to division in Figure 2M. Other divisions in the movie are also abnormally oriented, and what appears to be a new lumen forms towards the end of the recording. Left panel – merged image. Middle panel – Actin Chromobody, tagRFP. Right panel – Histone-2B-CFP. 4 frames/second.

The interaction between CASK and the tumour suppressor Dlg1 regulates mitotic spindle orientation in mammalian epithelia

Andrew P. Porter, Gavin R. M. White, Natalie A. Mack, and Angeliki Malliri

J Cell Sci 2019. 132:None-None; doi: 10.1242/jcs.230086