Movie 5

Arl3T31N: Pichia pastoris PpArl3T31N mutant strain expressing msGFP-VIG4 and SEC7-DsRed.M1x6 was grown to the log phase. Live cell 4D movies were captured using Leica SP8 with optical z slice 0.3nm, zoom factor 7 and 100x magnification. The representative 4D movie was deconvoluted using Huygens Pro software, and Z projected using ImageJ. Arrow marking the Golgi cisternal unstacking and stacking events. The time interval is in mm:: ss.