Timelapse video of cytoplasmic actin comet labeled with LifeAct::mKate (red) and LifeAct::GFP (cyan). Note lag of LifeAct::mKate signal relative to LifeAct::GFP yielding a cyan tip followed by a red tail. Elapsed time shown (sec). Scale bar, 2.5 μm. See related Figure 3G-H.