Movie 6

Mouse Tumor Cell Cluster Angiopellosis Extravasation and Proliferation Over 96 Hours. In vivo imaging of a cluster of injected fluorescently-labelled B16F10 murine melanoma cell cluster undergoing extravasation through angiopellosis. The HeLa cell cluster (cyan) is lodged in the lumen of blood vessel (green), prompting vascular remodeling. The tumor cell cluster is removed from the blood vessel into surrounding tissue as a multicellular group. 3D rendered version on right. The cell cluster is then imaged once every 24 hours post extravasation to monitor growth over 96 hours. SB = 50 μm.

Circulating tumor cells exit circulation while maintaining multicellularity, augmenting metastatic potential

Tyler A. Allen, Dana Asad, Emmanuel Amu, M. Taylor Hensley, Jhon Cores, Adam Vandergriff, Junnan Tang, Phuong-Uyen Dinh, Deliang Shen, Li Qiao, Teng Su, Shiqi Hu, Hongxia Liang, Heather Shive, Erin Harrell, Connor Campbell, Xinxia Peng, Jeffrey A. Yoder, and Ke Cheng

J Cell Sci 2019. 132:None-None; doi: 10.1242/jcs.231563