Movie 1

Ba/F3 cells were stably expressed N-terminally EGFP-tagged ATP11C-a (A and A’) or ATP11C-b (B and B’). ATP11C-b localized to the uropod at the cell posterior, whereas ATP11C-a localized to the entire plasma membrane including the pseudopods and uropod. Time-lapse images were recorded every 20 sec in the presence of the nuclear stain Hoechst33342 and movies correspond to 10 frames per second.

The cytoplasmic C-terminal region of the ATP11C variant determines its localization at the polarized plasma membrane

Masahiro Takayama, Hiroyuki Takatsu, Asuka Hamamoto, Hiroki Inoue, Tomoki Naito, Kazuhisa Nakayama, and Hye-Won Shin

J Cell Sci 2019. 132:None-None; doi: 10.1242/jcs.231720