Movie 1

Ba/F3 cells were stably expressed N-terminally EGFP-tagged ATP11C-a (A and A’) or ATP11C-b (B and B’). ATP11C-b localized to the uropod at the cell posterior, whereas ATP11C-a localized to the entire plasma membrane including the pseudopods and uropod. Time-lapse images were recorded every 20 sec in the presence of the nuclear stain Hoechst33342 and movies correspond to 10 frames per second.