Movie 2

Ba/F3 cells stably expressing N-terminally EGFP-tagged ATP11C-a and tRFP-tagged ATP11C-b. ATP11C-b localized to the uropod at the cell posterior, whereas ATP11C-a localized to the entire plasma membrane including the pseudopods and uropod. Timelapse images were recorded every 20 sec in the presence of the nuclear stain Hoechst33342 and movies correspond to 10 frames per second. TD, transmission differential interference contrast.