To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Movie 4 Representative TIRF-SIM images of live Jurkat CD4+ T-cells expressing Lifeact-citrine. The TCR is fluorescently labelled aCD3-Alexa568. The frame-rate is 1 fps. Scale bar is 5μm. Distinct actin cytoskeleton behaviour in primary and immortalised T-cells Huw Colin-York, Sudha Kumari, Liliana Barbieri, Lena Cords, and Marco Fritzsche J Cell Sci 2020. 133:None-None; doi: 10.1242/jcs.232322 Download Original Video