Movie 1

Epididymis from a V-ATPase B1-EGFP mouse. 3D reconstruction from a stack of 0.3-μm interval images, acquired using the Zeiss LSM800 confocal microscope. EGFP+ cells present long protrusions similar to “nanotubes” from their apical membrane. “Nanotubes” are in close proximity with sperm. DNA in sperm and epithelial cell is labeled in blue with DAPI.

Novel role of proton-secreting epithelial cells in sperm maturation and mucosal immunity

Maria A. Battistone, Raul German Spallanzani, Alexandra C. Mendelsohn, Diane Capen, Anil V. Nair, Dennis Brown, and Sylvie Breton

J Cell Sci 2020. 133:None-None; doi: 10.1242/jcs.233239