Epididymis from a V-ATPase B1-EGFP mouse. 3D reconstruction from a stack of 0.3-μm interval images, acquired using the Zeiss LSM800 confocal microscope. EGFP+ cells present long protrusions similar to “nanotubes” from their apical membrane. “Nanotubes” are in close proximity with sperm. DNA in sperm and epithelial cell is labeled in blue with DAPI.
Maria A. Battistone, Raul German Spallanzani, Alexandra C. Mendelsohn, Diane Capen, Anil V. Nair, Dennis Brown, and Sylvie Breton
J Cell Sci
2020. 133:None-None; doi: 10.1242/jcs.233239