Movie 10

Sperm-vesicle complex in epididymal sections from a B1-EGFP mouse. 3D reconstruction from a stack of 0.3-μm interval images corresponding to Fig. S1 bottom panels, acquired using the Zeiss LSM800 confocal microscope. EGFP+ vesicle in close interaction with the sperm head in the proximal region. DNA in sperm and epithelial cell is labeled in blue with DAPI.

Novel role of proton-secreting epithelial cells in sperm maturation and mucosal immunity

Maria A. Battistone, Raul German Spallanzani, Alexandra C. Mendelsohn, Diane Capen, Anil V. Nair, Dennis Brown, and Sylvie Breton

J Cell Sci 2020. 133:None-None; doi: 10.1242/jcs.233239