Sperm-vesicle interaction events in epididymal sections from a B1-EGFP mouse. 3D reconstruction from a stack of 0.3-μm interval images corresponding to Fig. S1 upper panels, acquired using the Zeiss LSM800 confocal microscope. EGFP+ vesicle in close interaction with the sperm head in the proximal region. Note that toward the end of the video, there is an EGFP-positive tube in close interaction with the head of another sperm. DNA in sperm and epithelial cell is labeled in blue with DAPI.
Maria A. Battistone, Raul German Spallanzani, Alexandra C. Mendelsohn, Diane Capen, Anil V. Nair, Dennis Brown, and Sylvie Breton
J Cell Sci
2020. 133:None-None; doi: 10.1242/jcs.233239