Movie 9

Sperm-vesicle interaction events in epididymal sections from a B1-EGFP mouse. 3D reconstruction from a stack of 0.3-μm interval images corresponding to Fig. S1 upper panels, acquired using the Zeiss LSM800 confocal microscope. EGFP⁺ vesicle in close interaction with the sperm head in the proximal region. Note that toward the end of the video, there is an EGFP-positive tube in close interaction with the head of another sperm. DNA in sperm and epithelial cell is labeled in blue with DAPI.