Movie 1

Spindle assembly, chromosome alignment, anaphase I and PBE in a control oocyte. Timelapse imaging of an oocyte expressing H2B-RFP (red) and stained with SiR-tubulin dye (green). Time scale is shown in hh:mm. Note that a bipolar spindle assembles slowly close to the centre of the oocyte prior to migrating to the oocyte cortex and undergoing anaphase I around 9 h post-GVBD followed accompanied very shortly after by PBE.