Movie 10

The Wee1 inhibitor, MK1775, prevents anaphase I and PBE in control oocytes. Timelapse imaging of a control oocyte expressing H2B-RFP (red) and stained with SiR-tubulin dye (green) treated with MK1775 from 6 h post-GVBD. Time scale is shown in hh:mm. Note that anaphase I and PBE do not occur even by 15 h post-GVBD.