Movie 2

Spindle assembly and chromosome alignment without anaphase I in a Cep55-depleted oocyte. Timelapse imaging of a Cep55-depleted oocyte expressing H2B-RFP (red) and stained with SiR-tubulin dye (green). Time scale is shown in hh:mm. Note that a bipolar spindle assembles and chromosomes align normally but that anaphase I fails to occur even by 19 h post-GVBD.