Movie 5

Spindle assembly, chromosome alignment, cyclin B1 proteolysis, anaphase I and PBE in a mock-depleted oocyte. Timelapse imaging of an oocyte expressing H2B-RFP (red) and cyclin B1-GFP (monochrome) and stained with SiR-tubulin dye (green). Time scale is shown in hh:mm. Note that a bipolar spindle assembles, chromosomes align and anaphase I occurs around 9 h post-GVBD concurrently with which, cyclin B1-GFP levels reach a nadir followed very shortly after by PBE, entry into MII and partial recovery of cyclin B1-GFP levels.