Movie 6

Spindle assembly, chromosome alignment and cyclin B1 proteolysis without anaphase I or PBE in a Cep55-depleted oocyte. Timelapse imaging of an oocyte expressing H2B-RFP (red) and cyclin B1-GFP (monochrome) and stained with SiR-tubulin dye (green). Time scale is shown in hh:mm. Note that a bipolar spindle assembles, chromosomes align and securin-GFP reaches a nadir around 8.5 h post-GVBD followed by partial recovery but that neither anaphase I nor PBE occur.