Movie 7

Forced Cdk1 inactivation by flavopiridol induces anaphase I and PBE in Cep55-depleted oocytes. Timelapse imaging of a Cep55-depleted oocyte expressing H2B-RFP (red) and stained with SiR-tubulin dye (green) treated with flavopiridol from 6 h post-GVBD. Time scale is shown in hh:mm. Note that anaphase I occurs by 30 min following treatment and is followed by PBE that is apparent by 1.5 hours. Note also that chromosomes eventually decondense due to persistent Cdk1 inactivation induced by flavopiridol.