Movie 11

Comparison of CCCP-induced circularization in control and Oma1 KD U2OS cells
Control cells (scrambled siRNA) were transfected with mito-Ds-Red (green), and Oma1 KD were transfected with mito-BFP (red). At 5 hr after transfection, both types of cell were re-plated on the same coverslip and allowed to adhere for 18 hours prior to imaging. A field containing both red and green cells was located, and confocal images acquired every 15sec. 20μM CCCP was added at time 00:00 min:sec. Corresponds to Figure 5G. Scale bar: 10μm