Movie 3

Ionomycin-induced actin polymerization in U2OS cells - medial region.
U2OS cell expressing Ftractin-GFP (green) and mito-BFP (red), stimulated with 4μM Ionomycin at time 0 (sec). Confocal images acquired every 15sec at medial region (2-4 μm above basal surface). Box denotes the region in inset at right. Corresponds to Figure S1B. Scale bar: 10μm for whole cell and 5μm for inset.