Movie 5

Transient depolarization and actin polymerization in U2OS cells.
U2OS cell, pre-treated with DMSO for 30 min, co-transfected with Ftractin-GFP (green) and mito-BFP (red), and stained with TMRE (cyan). Transient depolarization occurs at time 0 (sec). Confocal images acquired every 1.2sec in the medial region. * denotes the region in inset at right. Corresponds to Figure 2A. Scale bar: 10μm for whole cell and 5μm for insert. Cell is the DMSO-treated control for the CK666 treatment in Movie 6.