Movie 7

Examples of mitochondrial circularization after CCCP treatment
WT U2OS cells transfected with Mito-DsRed (red) and stimulated with 20μM CCCP at time 0 (sec). Examples show: (A) matrix rearrangement at a branch point, (B) matrix rearrangement at the center, (C) matrix rearrangement at the end of a mitochondrion, and (D) curling of the mitochondrion to form a circle. Confocal images acquired every 4sec at the basal region. Playback: for A, B and D, 4 sec/frame; for C, 16sec/frame. Corresponds to Figure 3A. Scale bar: 2μm.