To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Movie 5 Example VAMP72 HMM predicted active trajectory in protoplast with no co-localization. Blue line indicates HMM predicted active trajectory. Red channel in 3mCherry-VAMP72 and green channel is 3mEGFP-myosin XIa. In vivo interactions between myosin XI, vesicles and filamentous actin are fast and transient in Physcomitrella patens Jeffrey P. Bibeau, Fabienne Furt, S. Iman Mousavi, James L. Kingsley, Max F. Levine, Erkan Tüzel, and Luis Vidali J Cell Sci 2020. 133:None-None; doi: 10.1242/jcs.234682 Download Original Video