Movie 1

Knocksideways of DNM2-GFP using 3xFKBP Dongle.
Live cell confocal microscopy of dongle-knocksideways, 200 nM rapamycin is added at 10 s. Dynamin-2-GFP (left, green) and MitoTrap (middle, red) are shown together with a merge (right). Time, seconds. Scale bar, 10 μm.

Unintended perturbation of protein function using GFP nanobodies in human cells

Cansu Küey, Gabrielle Larocque, Nicholas I. Clarke, and Stephen J. Royle

J Cell Sci 2019. 132:None-None; doi: 10.1242/jcs.234955