Movie 1

Live-imaging of GDE2 intracellular trafficking in SH-SY5Y cells. A single cell expressing eGFP-tagged GDE2 was imaged on a Leica SP8 confocal microscope using a 63x oil immersion objective at ~8 frames per min. Note the rapid motility of vesicular GDE2 and its bi-directional movement towards and from the neurite ending. Scale bar and timing as indicated. Image size, 81 x 33 μm.