Movie 1

Antigen is transported in vesicles positive for early and late endosomal markers. A20 D1.3 cells were transfected with GFP-Rab5 (right panel), loaded with LysoTracker (left panel) and activated with RRx-αIgM (middle panel). Live-imaging was performed with SDCM (sCMOS Orca Flash4 v2 camera) on a single plane. Triple-positive vesicles are highlighted with a purple circle tracking the spots in the antigen channel. Movie was recorded 10 min after activation and imaged every 2 s.

B cells rapidly target antigen and surface-derived MHCII into peripheral degradative compartments

Sara Hernández-Pérez, Marika Vainio, Elina Kuokkanen, Vid Šuštar, Petar Petrov, Sofia Forstén, Vilma Paavola, Johanna Rajala, Luqman O. Awoniyi, Alexey V. Sarapulov, Helena Vihinen, Eija Jokitalo, Andreas Bruckbauer, and Pieta K. Mattila

J Cell Sci 2020. 133:None-None; doi: 10.1242/jcs.235192