Movie 2

Antigen colocalises with acidic vesicles soon after internalisation. A20 D1.3 cells were loaded with LysoTracker (middle panel) and activated with AF488-αIgM (left panel). Right panel shows merge image of antigen channel (magenta) and LysoTracker channel (cyan). Liveimaging was performed with SDCM (EVOLVE camera) on a single plane every 500 ms. Doublepositive vesicles are highlighted with a purple circle tracking the spots in the antigen channel. Movie was recorded 1 min after cell activation.

B cells rapidly target antigen and surface-derived MHCII into peripheral degradative compartments

Sara Hernández-Pérez, Marika Vainio, Elina Kuokkanen, Vid Šuštar, Petar Petrov, Sofia Forstén, Vilma Paavola, Johanna Rajala, Luqman O. Awoniyi, Alexey V. Sarapulov, Helena Vihinen, Eija Jokitalo, Andreas Bruckbauer, and Pieta K. Mattila

J Cell Sci 2020. 133:None-None; doi: 10.1242/jcs.235192