Movie 10

MbsN300 overexpressing LEC showing impaired contractile behaviour. Detail confocal micrographs of a LEC during early constriction. F-actin labelled with GMA-GFP. Cell only shows rudimentary pulsatile behaviour of a not very dynamic apicomedial network, which does not generate clear foci, but only shows some diffuse activity, which moves around the apicomedial network. Scale bar, 10 μm; a, anterior; p, posterior; m, medial (corresponds to dorsal); l, lateral (corresponds to ventral).