Movie 12

Rok-CAT overexpression in LECs at 29°C, ‘strong’ phenotype. F-actin labelled with GMA-GFP. LECs are merely constricting, showing extensive blebbing (pink arrowheads) and cortical actin bundles (orange arrowheads). No pulsatile behaviour visible. Eventually, cells delaminate (red arrow). Scale bar, 10 μm; a, anterior; p, posterior; m, medial (corresponds to dorsal); l, lateral (corresponds to ventral).