Movie 13

Rho1-CA overexpression in LECs, ‘migrating’ phenotype. F-actin labelled with GMA-GFP. Beginning of morphogenesis, migration and constriction visible. LECs migrate, showing lamellipodia (cyan arrowheads); constricting LECs show blebbing (pink arrowheads). No pulsatile behaviour visible. Scale bar, 10 μm; a, anterior; p, posterior; m, medial (corresponds to dorsal); l, lateral (corresponds to ventral).