Movie 14

Rho1-CA overexpression in LECs, ‘non-migrating’ phenotype. Apical (left) and apicolateral (right) z-slice shown. F-actin labelled with GMA-GFP. LECs are merely constricting, showing extensive blebbing (pink arrowheads). No pulsatile behaviour visible. Eventually, cells delaminate (yellow arrow). Scale bar, 10 μm; a, anterior; p, posterior; m, medial (corresponds to dorsal); l, lateral (corresponds to ventral).