Rho1 overexpression in LECs. F-actin labelled with GMA-GFP. Orange asterisks indicate LECs that cycle between (1) the presence of apicomedial actin and (2) the absence of apicomedial actin but increased junctional cortical actin and blebbing. Pink arrowhead highlights LEC which is blebbing and shows junctional cortical actin. Scale bar, 20 μm. Anterior is to the left.