Movie 2

Different phases of LEC behaviour. Confocal micrographs of LECs labelled with GMA-GFP. During early migration (phase 1), LECs migrate without showing pulsed contractions. Merely flickering of apical activity is visible. During late migration (phase 2), LEC shows two actin foci alternating in their back (red dots). During constriction (phase 3), LEC shows an individual central actin focus (yellow dot). Cyan arrowheads, lamellipodium; hb, histoblasts; scale bar, 10 μm; a, anterior; p, posterior; m, medial (corresponds to dorsal); l, lateral (corresponds to ventral).