Movie 3

During dorsal repolarisation of a LEC, pulsatile activity reorganises to the back of the dorsally-migrating cell. Confocal micrographs of LECs labelled with GMA-GFP. Before repolarisation, actin focus is in the cell centre (1st red dot). After repolarisation, actin focus is found in the back of the cell (2nd red dot). hb, histoblasts; scale bar, 10 μm; a, anterior; p, posterior; m, medial (corresponds to dorsal); l, lateral (corresponds to ventral).