Movie 6

Overview movie of Rok-RNAi LECs showing impaired contractile behaviour and actin flows in the back of the cells. Overview confocal micrographs of LECs during late migration and constriction, F-actin labelled with GMA-GFP. LECs generate a lamellipodium in the front (cyan arrowheads) and show an apicomedial network, which does not generate clear foci, but only shows some diffuse activity (e.g. in area highlighted by yellow ellipsoid). Some cells create contractile flows in their back (cyan asterisks). LECs constrict and delaminate eventually (red arrow). Scale bar, 10 μm; a, anterior; p, posterior; m, medial (corresponds to dorsal); l, lateral (corresponds to ventral).