Movie 1

Time-lapse microscopy analysis of N4-Dendra2 uptake by N1-mCherry-expressing cells. A431D cells expressing N1-mCherry (red, endosomal localization) were co-cultured with A431D cells expressing N4-Dendra2 (green, plasma membrane localization). Cells were imaged on the second day after plating. Green and red images were taken simultaneously using an image splitter in 1 min intervals using halogen light that minimized phototoxicity and photobleaching and are shown at 2 images per second. White arrows indicate green fluorescence moving from the plasma membrane of N4 cells into N1 cells. Yellow color visualizes co-localization of green and red signals.

Trans-endocytosis elicited by nectins transfers cytoplasmic cargo, including infectious material, between cells

Alex R. Generous, Oliver J. Harrison, Regina B. Troyanovsky, Mathieu Mateo, Chanakha K. Navaratnarajah, Ryan C. Donohue, Christian K. Pfaller, Olga Alekhina, Alina P. Sergeeva, Indrajyoti Indra, Theresa Thornburg, Irina Kochetkova, Daniel D. Billadeau, Matthew P. Taylor, Sergey M. Troyanovsky, Barry Honig, Lawrence Shapiro, and Roberto Cattaneo

J Cell Sci 2019. 132:None-None; doi: 10.1242/jcs.235507