Movie 3

Representative movies of embryos injected with RNAs transcribed from constructs with small deletions (numbering #1-#9 according to Fig. 3A and Suppl. Table 1 for details of the constructs) (left → right): gfp-del [392-441] crb 3’UTR-sv40 (#1), gfp-del [417-466] crb 3’UTRsv40 (#2), gfp-del [442-491] crb 3’UTR-sv40 (#3), gfp-del [467-516] crb 3’UTR-sv40 (#4), gfpdel [492-538] crb 3’UTR-sv40 (#5), gfp-del [517-570] crb 3’UTR-sv40 (#6), gfp-del [542-590] crb 3’UTR-sv40 (#7) and gfp-del [566-662] crb 3’UTR-sv40 (#8) in that order. RNAs from constructs 4, 5, 6, 7 do not show apical localisation suggesting that the region covered by these deletions contains the LE. Arrows in the rest of the movies mark the apical localisation of remaining constructs.