Movie 5

Representative movies of embryos injected with mutations introduced into the construct gfp-[499-545] crb 3’UTR-sv40. These mutations affect the key features of the LE required for its possible recognition and transport by Egl (see Fig. 3E). The following mutations were tested: (a) C-G replaced by U-A; (b) C-G replaced by U-A; (c) both C-G pairs (a,b) replaced by U-A pairs; (d) U-A base pair replaced by A-U; Base pairs in brackets have been deleted: (e) both U-A and A-U base pairs deleted; (f) only the U-A base pair deleted. RNA transcribed from constructs (a-e) show no apical localisation. Construct (f) shows apical localisation as indicated by the white arrow. Scale bar: 5μm.