Movie 2

formation and propagation of actin arcs.
This movie shows the dynamic of actin network (Lifeact-GFP) in RPE1 cells plated on crossbow-shaped micropattern. Cells were treated with a control siRNA (siCTR, left) or siRNAs against α-actinin 1 and 4 (siACTN, right). Images were taken every minute. The inset on the right is a magnified view of the upper-right part of the cell. Actin arcs formed in retracting protrusions and concatenated in a continuous cable lining the cell front as they contracted and moved inward.

Spatial integration of mechanical forces by α-actinin establishes actin network symmetry

Fabrice Senger, Amandine Pitaval, Hajer Ennomani, Laetitia Kurzawa, Laurent Blanchoin, and Manuel Théry

J Cell Sci 2019. 132:None-None; doi: 10.1242/jcs.236604