Movie 9

Mitotic divisions in an anti-Mora injected expressing Eb1-GFP. Imaging begins before NEB, shows a mitotic division of cycle 11 from prophase to telophase. Note that while Eb1-GFP is loaded at center of asters in interphase like in control (movie 8). However, following NEB, Eb1-GFP recruitment at growing MT plus ends is dramatically delayed and distinct comet tracks are lacking. Stills of this movie are shown in Figure 4G.