Mitotic divisions in an anti-Mora injected expressing Eb1-GFP. Imaging begins before NEB, shows a mitotic division of cycle 11 from prophase to telophase. Note that while Eb1-GFP is loaded at center of asters in interphase like in control (movie 8). However, following NEB, Eb1-GFP recruitment at growing MT plus ends is dramatically delayed and distinct comet tracks are lacking. Stills of this movie are shown in Figure 4G.
Valeria Palumbo, Ammarah Tariq, Lori Borgal, Jeremy Metz, Mara Brancaccio, Maurizio Gatti, James G. Wakefield, and Silvia Bonaccorsi
J Cell Sci
2020. 133:None-None; doi: 10.1242/jcs.236786