Movie 2. Ezrin from the site of phagocytosis during phagocytosis

The movie shows the complete experiment from which the data in fig 6 was constructed. Three movie sequences of images of a RAW 264.7 cell expressing C-terminally tagged ezrin-GFP and loaded with the Ca2+ indicator fura-red are shown. The first sequence shows the phase contrast images (PContrast): the second the ezrin distribution; and the third the fura red intensity which decreases with an elevation of cytosolic Ca2+.

Ca2+-activated cleavage of ezrin visualised dynamically in living myeloid cells during cell surface area expansion

Rhiannon E. Roberts, Marianne Martin, Sabrina Marion, Geetha L. Elumalai, Kimberly Lewis, and Maurice B. Hallett

J Cell Sci 2020. 133:None-None; doi: 10.1242/jcs.236968