Movie 2

Video illustrates application of macrophage binary mask over the entire course of live cell time-lapse high-content microscopy imaging. Time-lapse video is the same as shown in Movie 1, but also contains an overlay of the macrophage binary mask manually determined with NIS-Elements software and then applied over all frames (green). Individual frames 1 (0 min) and 40 (~60 min) are depicted in Fig1D.

High-resolution quantification of discrete phagocytic events by live cell time-lapse high-content microscopy imaging

Charles C. Chu, Jonathan J. Pinney, Hannah E. Whitehead, Fatima Rivera-Escalera, Karl R. VanDerMeid, Clive S. Zent, and Michael R. Elliott

J Cell Sci 2020. 133:None-None; doi: 10.1242/jcs.237883