Video close-up of only target cell dye-label channel during phagocytosis with live cell time-lapse high-content microscopy imaging illustrates lack of initial sensitivity of dye uptake measure. Time-lapse video is same close-up region as shown in Movie 3, except only CypHer5 (APC) channel is displayed. Individual frames 1 (0 min), 20 (~30 min), 40 (~60 min), and 60 (~90 min) are depicted in Fig.2A CypHer5.