Movie 9

Video close-up illustrates the lack of target dye (pHrodo Red) intensity increase following engulfment when macrophages are pre-treated with BafA1. Time-lapse video is same close-up region as shown in Fig. 4A (Bottom) and includes the merged CTDR (macrophage) and pHrodo Red (target) channels. Movie starts at ~39 minutes due to delay between capture of background frames and addition of antibody and targets.

High-resolution quantification of discrete phagocytic events by live cell time-lapse high-content microscopy imaging

Charles C. Chu, Jonathan J. Pinney, Hannah E. Whitehead, Fatima Rivera-Escalera, Karl R. VanDerMeid, Clive S. Zent, and Michael R. Elliott

J Cell Sci 2020. 133:None-None; doi: 10.1242/jcs.237883