Video close-up illustrates the lack of target dye (pHrodo Red) intensity increase following engulfment when macrophages are pre-treated with BafA1. Time-lapse video is same close-up region as shown in Fig. 4A (Bottom) and includes the merged CTDR (macrophage) and pHrodo Red (target) channels. Movie starts at ~39 minutes due to delay between capture of background frames and addition of antibody and targets.