Movie 2
Search and kill assays of cognate targets in the presence of Lck and Mek 1/2 inhibition
Live-cell imaging of LGO1 cells (green) co-embedded with cognate EL-4 tumour cells (blue) in a 3D collagen matrix over 12 h in the presence of vehicle (left), 10 μM Lck inhibitor (middle) and 5 μM Mek 1/2 inhibitor (right). Killed cells appear in pink (propidium iodide uptake). Time in h:min:s. Scale bar: 100μm
The Lifeact-EGFP mouse is a translationally controlled fluorescent reporter of T cell activation
J Cell Sci 2020. 133:None-None; doi: 10.1242/jcs.238014