Movie 4

Identification of T cells in 3D imaging data in a non-cognate environment
Live-cell imaging of LGO1 cells (raw fluorescence, left; coloured surface rendering, right) co-embedded with non-cognate EL-4 tumour cells (not shown) in a 3D collagen matrix over 12 h. Surfaces coloured according to activation confidence level as described in Fig. 5A: grey: indeterminate, yellow: low, cyan: medium, magenta: high. Time in h:min:s. Scale bar: 100μm